Mitotracker intensity
Web16 apr. 2013 · This approach allows for a correlation of intensity peaks that mirrors colocalization of fluorescent molecules. In this example, it is possible to see the dual LAMP1-GFP (green) peaks representing the lysosomal membrane and the single MitoTracker ® Red peak illustrating the presence of a mitochondrion in a lysosome. Web18 mei 2011 · MitoTracker Green, a probe used to evaluate mitochondrial mass, is a P-gp substrate, and its staining profile is dependent on the activity of this protein. Many factors, such as P-gp expression or activity, alterations in mitochondrial lipid and protein content, mitochondrial ABC protein expression or MDR phenotype, can lead to a misinterpretation …
Mitotracker intensity
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WebMitoTracker Deep Red FM is a far red-fluorescent dye (abs/em 644/665 nm) that stains mitochondria in live cells and can be used for mitochondrial localization. The dye is well-retained after aldehyde fixation and even subsequent permeabilization with detergents. Web1 jun. 2004 · To determine mitochondrial potential during transport, neurons were stained with MitoTracker Green FM (Molecular Probes) at a final concentration of 500 nM in medium for 20 minutes and then incubated for 30 minutes in fresh medium.
Web15 jan. 2001 · MitoTracker Orange showed the most robust increase in fluorescence intensity in neurons and this was observed at concentrations of 50–500 nM. A slow, delayed decrease in fluorescence was observed during the wash period, indicating either re-sequestration as mitochondria repolarize or a leakage of the dye from the cell ( Fig. 2 B,E). Web24 jan. 2024 · Since the MCU has a low affinity for Ca 2+, this limits the amount of Ca 2+ uptake during diastole and/or Ca 2+ transients when at rest ( Andrienko et al., 2009 ). It is estimated that about 1% of cytosolic Ca 2+ released during the Ca 2+ transient is taken up by the MCU ( Bassani et al., 1992 ).
Web29 mrt. 2012 · The 160 highest scoring compounds that increased MitoTracker intensity after normalizing for cell size were selected for secondary assays. The assays consisted of immunofluorescence assays for mitochondrial protein content and qPCR assays for mitochondrial DNA (mtDNA) copy number. WebMitotracker intensity evolution during the erythroid differentiation process. Shown is the distribution of the mitotracker intensity at each time-point of the differentiation process …
Web1 jan. 2024 · We analysed the mean fluorescence intensity (MFI) of vehicle (PBS) and LPS-treated macrophages for MitoTracker Green probe and observed that, even …
Web12 apr. 2024 · For mitochondrial and lysosomal motility 25 nM MitoTracker Red CMXRos (Invitrogen, #M7512), and 25 nM LysoTracker red (Invitrogen, #L7528) ... In the rescue experiment, vimentin distribution was quantified using CellProfiler 4.2.1 29 module object intensity distribution. cvs vaccine scheduler covidWeb1 aug. 2011 · INTRODUCTION Membrane-potential-dependent dyes such as Rhodamine 123, tetramethylrhodamine methyl ester (TMRM), and tetramethylrhodamine ethyl ester (TMRE) are useful as long as the mitochondrion maintains its negative membrane potential. MitoTracker is a commercially available fluorescent dye (Invi … rain valleyWeb30 mrt. 2016 · Our data also suggest that the intensity of MitoTracker Deep Red changes along with mitochondrial potential. Mitochondrial … rain vape juice