Cytofectins

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August undefined, 2024

WebCationic lipids (cytofectins) have gained widespread acceptance as pharmaceutical polynucleotide delivery agents for both cultured cell and in vivo transfection, and the cytofectins DOTAP and DC ... WebSep 28, 2008 · Download Citation Cationic Lipid/Polynucleotide Condensates for in Vitro and in Vivo Polynucleotide Delivery - the Cytofectins Abstract Cationic liposomes have …

Liposomal Formulation of Monovalent Cholesteryl Cytofectins …

WebCompositions and methods of protecting aquatic invertebrates from disease is shown. In one embodiment, dsRNA or antisense RNA to a nucleic acid molecule of the disease-causing mic Webionic lipids (cytofectins) have been used in many lipid-DNA formulations, and since the report of the cytofectin DOTMA2 which is often used with the neutral lipid dioleoyl L-α-phosphatidylethanolamine (DOPE) (Fig. 1), a range of related lipids have been described.1e,3 The aim in developing new cytofectins has been to optimise the delivery ... dunkirk theme tune

Novel cationic amphiphiles as delivery agents for antisense ...

Webproduce cytofectins which afford liposomes with high transfection potential, the nature of the linkage, spacer length and cationic head group have been examined (Figure 1). Thus, it has been shown that the ether linkage between the hydrophobic cholesteryl skeleton and the spacer affords compounds which lead to higher transfection ... WebA large number of liposome-based non-viral gene and siRNA delivery systems include monovalent cholesteryl cytofectins with acyclic head groups in their formulations. Progress in their clinical development has, however, been hampered by relatively low transfection efficiencies. WebThis invention relates to the field of biotechnology or genetic engineering. Specifically, this invention relates to the field of gene expression. More specifically, this inventio dunkirk technical support

Structural and functional analysis of cationic ... - Europe PMC

Synergy between cationic lipid and co-lipid determines the …

WebSummary DNA, depending on base sequence, can induce a wide range of immune responses. While bacterial DNA is stimulatory, mammalian DNA is inactive alone and can, moreover, inhibit the response to ... WebJun 28, 2015 · A large number of liposome-based non-viral gene and siRNA delivery systems include monovalent cholesteryl cytofectins with acyclic head groups in their formulations. Progress in their clinical... dunkirk the bookWebSep 17, 1996 · Amine containing compounds and their use in the generation of cytofectin:polynucleotide complexes for transfection of cells, formulations, counterions, and reaction conditions for maximizing the transfection include using cationic amine compounds that have the general structure: ##STR1## wherein R.sub.4 and R.sub.5 are a pair of … dunkirk the mole meaning

"WebFeb 15, 2002 · Anchor-dependent lipofection with non-glycerol based cytofectins containing single 2-hydroxyethyl head groups Detailed structure-activity investigations aimed at probing the anchor chain length dependency for glycerol-based lipofectins have been reported previously. " - Cytofectins

Cytofectins

Mono- and dicationic short PEG and methylene ... - PubMed

WebFeb 1, 1996 · Cationic lipids (cytofectins) have gained widespread acceptance as pharmaceutical polynucleotide delivery agents for both cultured cell and in vivo transfection, and the cytofectins DOTAP and DC-Cholesterol are being tested in clinical human gene therapy trials. This study reports the effects of modifications in the hydrophobic domain … WebJun 26, 2010 · Whilst constituent cytofectins may be basic enough to forge cohesive electrostatic interactions with the cargo DNA, strong binding may also be associated with a drop in transfection activity through poor dissociation after cell entry. Similarly, the protective effect afforded to lipoplexes by pegylation must be weighed against the drop in ...

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WebDec 21, 2001 · We report herein the design, synthesis, and transfection biology of a novel series of non-glycerol-based cationic lipids with lactic acid-derived head groups The … WebMay 24, 2024 · Cytotoxic drugs can kill cells or stop them from growing and dividing. Genotoxic drugs cause damage to the DNA inside the cells. Carcinogens damage DNA in cells. This causes mutations that can lead …

WebCefoxitin is an antibiotic used to treat a wide variety of bacterial infections. It may also be used before and during certain surgeries to help prevent infection. This medication is known as a ... WebApr 4, 2002 · Both of these cytofectins are predicted to produce relatively fluid bilayer liposome structures due to the short alkyl side chain of GAP-DLRIE (C12:0) and the mono-unsaturated C14 side chain of GAP-DMORIE (C14:1). In addition, the co-lipid used in the lipoplex formulations is also important, and thus structural differences in either or both ...

WebNov 1, 2005 · Novel N,N‘-diacyl-1,2-diaminopropyl-3-carbamoyl[bis-(2-dimethylaminoethane)] bivalent cationic lipids were synthesized and evaluated for in … WebA large number of liposome-based non-viral gene and siRNA delivery systems include monovalent cholesteryl cytofectins with acyclic head groups in their formulations. Progress in their clinical development has, however, been hampered by relatively low transfection efficiencies. Structural differences between members of this class of cationic amphiphiles …

WebHere, two homologous cationic cholesteryl cytofectins 3 beta[N-(N',N'-dimethylaminoethane)-carbamoyl] cholesterol (DC-Chol) and 3 beta[N-(N',N'-dimethylaminopropane)-carbamoyl] cholesterol (Chol-T) with 2 and 3 carbon spacer elements, respectively, have been formulated into liposomes with near equimolar …

WebBoth single-stranded and double-stranded DNAs were active in the presence of cytofectins. Macrophage activation by the DNA-cytofectin complexes was reduced by chloroquine, … dunkirk theatersWebTo evaluate additional delivery methods for the gene, Genzyme has an exclusive research agreement for the use of Vical's cytofectins as non-viral delivery vectors for CFTR. Also under investigation are delivery systems for the nasal epithelium using liposomes or lipid-DNA complexes. dunkirk the story behind the legendWebFeb 1, 2008 · Two novel cholesterol-based cytofectins containing primary amino head groups, glycylcholesteryl formylhydrazide (MS10) and beta-alanylcholesterylformylhydrazide (MS11), have been prepared and incorporated into unilamellar cationic liposomes in equimolar amounts with dioleoylphosphatidylethanolamine (DOPE) as colipid. dunkirk veterans association badgeWebsystems, including cytofectins, can substantially increase the delivery of antisense compounds to cells, as well as the con-sequent pharmacological effects (6,7). Thus it is … dunkirk to rosslare ferry timeWebProvided is a collection of cytofectins (cytofectins are defined as chemical species that are cationic transfection amphiphiles) or cationic lipids that bind and transport … dunkirk warranty registrationWebAug 1, 1999 · Cytofectin complexes. Complexes of oligonucleotides with Lipofectin® or Lipofectamine® were prepared as previously described ( 6 ), essentially according to the manufacturer's directions. Plasmid transfection assay For transfections, ECV304 or MDR3T3 cells were seeded in 6-well dishes ∼24 h prior to transfection and used at … dunkirk things to doWebA large number of liposome-based non-viral gene and siRNA delivery systems include monovalent cholesteryl cytofectins with acyclic head groups in their formulations. … dunkirk water boiler troubleshooting