Cisplatin cck8

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August undefined, 2024

Web瑞禧生物提供科研级cisplatin-PEG-NPC顺铂-聚乙二醇-硝基苯碳酸盐,我们一直致力于研发纳米靶向科研产品以满足国内广大科研院校及其他相关科研单位的需求。cisplatin-PEG-NPC顺铂-聚乙二醇-硝基苯碳酸盐试剂不能用于人体治疗、药物开发、和其他商业用途。如果需要采购cisplatin-PEG-NPC顺铂-聚乙二醇-硝基 ... WebJan 1, 2024 · New therapy targets are urgently needed to reverse DDP resistance. Methods: The protein levels of these molecules were analyzed by Western blotting and immunofluorescent staining. Cell proliferation was detected by CCK8 and MTT assays. Cell migration and invasion were analyzed by wound healing assay and Transwell assays.

Cisplatin (Cisplatin Injection): Uses, Dosage, Side Effects

WebSep 15, 2024 · Cisplatin Injection (cisplatin injection (cisplatin (cisplatin injection) injection) ) is a sterile aqueous solution, available in 50, 100 and 200 mL multiple dose … WebApr 9, 2024 · Then cells were treated with cisplatin as indicated concentrations. 24 or 48 h later, 10 μL CCK8 reagent (HY-K0301, MedChemExpress, USA) was added into each well for another 2 h. Then the absorbance was measured at 490 nm using Cytation™ 5 Cell Imaging Multi-Mode Reader (Bio Tek). irc f6713sg 取扱説明書

(PDF) HMG20A was identified as a key enhancer driver associated …

Web Construction of cisplatin-resistant Siha cell line. (A) Morphology of Siha-N and Siha-DDP. (Magnifications, 200×). (B) Cell viability under the increasing concentration of cisplatin … WebCCK8 assays Cell viability was measured by cell counting kit-8 (CCK8). The cells were seeded into 96-well plates with 10 μL of CCK8 reagent in every well, and there were 4 × 104 cells in each well, all incubated at 37°C for 1 h. The optical density (OD) values were measured at 450-nm wavelength by Multiskan FC (Thermo Fisher Scientific, Inc.). WebFeb 16, 2024 · Generic name: cisplatin [ sis-PLA-tin ] Brand names: Platinol, Platinol-AQ, Platinol (Restricted Access) Dosage forms: intravenous powder for injection (50 mg), … order by in angularjs

Frontiers Transcriptional Activation of Gstp1 by MEK/ERK …

Cisplatin-resistant HeLa cells are resistant to apoptosis via p53 ...

WebCisplatin (CP) is a broad-spectrum antineoplastic agent, used to treat many different types of malignancies due to its high efficacy and low cost. However, its use is largely limited by acute... WebSep 30, 2024 · These cell strains were used to determine the effect of HOXB7 on cell viability, and cell apoptosis after exposure to cisplatin. CCK8 assay showed that treatment for 24 hours with cisplatin resulted in decreased IC50 of HOXB7‐knockdown cells compared with control cells (Fig (Fig3a). 3 a). This means that knockdown of HOXB7 … order by in access form irc f341sg f

"WebCisplatin-induced acute kidney injury in mice was established by intraperitoneal injection of cisplatin. The levels of urea nitrogen and creatinine in serum were measured， renal pathology change was assayed by HE staining， qRT-PCR and Western blot were used to detect the expression levels of PIM1 mRNA and PIM1 protein. " - Cisplatin cck8

Cisplatin cck8

HOXB7 mediates cisplatin resistance in esophageal squamous cell ...

WebWe established sublines that are resistant to cisplatin from EGFR wild-type cells (A549 and H460) and EGFR mutant cells (PC-9 and HCC827). The EGFR-related signals were examined by Western blotting. MTT assay and the trypan blue exclusion method were used for the in vitro study, while tumor size and the SUV of the 18FDG-PET scans were … WebCCK8 assay was performed to assess cell proliferation and chemosensitivity of cisplatin-resistant A549/DDP and H1299/DDP cells. Colony-forming assay was utilized to detect colony numbers. Cell migration and invasion ability were measured by transwell assay. The protein levels of LRP, Pgp, MRP1, and SIX1 were examined by Western blot assay.

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WebSep 30, 2024 · Cisplatin was the first platinum-based agent shown to have anti-tumor properties. 2 Cisplatin-based combinations remain first-line therapy for a number of solid tumors including lung, ovarian, and esophageal cancer. 3 It induces DNA damage through DNA cross-linking, thus interfering with the fundamental processes of DNA replication … WebCisplatin is a chemotherapy drug which is used to treat cancers including: sarcoma, small cell lung cancer, germ cell tumors, lymphoma, and ovarian cancer. While it is often …

WebMar 27, 2024 · Cisplatin-induced cell killing was analyzed by CCK8 assay. Intracellular ROS levels were detected by fluorescence-based flow cytometry analysis. In vitro overexpression and knockdown of GPX1 expression were performed using GPX1 expression vector and siRNA approaches. WebObjective: To investigate the regulatory effect of miR-29b on gastric cells' resistance to cisplatin. Methods: The expression of miR-29b in gastric cancer cell line treated with cisplatin concentration gradient was detected using quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) and Western blotting. CCK8 was used to …

Web目的探讨姜黄挥发油(TVO)联合顺铂对人皮肤鳞状细胞癌(鳞癌)A431细胞增殖、凋亡的影响及机制.方法取对数生长期A431细胞,用5、10、20、40、80 mg/L TVO处理,对照组用含有1％二甲基亚砜(DMSO)的DMEM高糖培养基处理,24 h后,CCK8法检测细胞增殖情况.另取部分A431细胞,分为4组,分别用含1％ DMSO的DMEM高糖培养基(对照 ... WebCisplatin (DDP) has been a significant tumor chemotherapy against various solid malignant tumors, ... Cell counting kit-8 assay. Cell viability was measured using the CCK-8 (SAB Biotech., College Park, MD, USA) assay. Cells were cultured in 96-well plates at 3 × 10 3 cells/well for 12 h. Following treatment for 48 h, the CCK-8 reagent was ...

WebJul 4, 2024 · To investigate whether miR‐320a regulates laryngeal carcinoma cell cisplatin resistance, miR‐320a overexpression HEp‐2 cells were treated with various concentration of cisplatin. CCK8 assays demonstrated that miR‐320a overexpression significantly inhibited HEp‐2 cell viability (Figure 4 E).

WebMethods: We use CCK8 assay to show synergistic cytotoxicity of COE and cisplatin. Then, PI single staining and FITC-Annexin V/PI double staining were used to observe apoptotic cells through flow cytometry. The proteins of caspase signaling pathway were examined by Western blotting. irc f6713sgWebNov 5, 2024 · Transcription factor knockdown and control cells were treated with cisplatin. CCK8 was used to detect cell viability and proliferation. Western blotting was … irc f1672dxWebExosome-transmitted circVMP1 facilitates the progression and cisplatin resistance of non-small cell lung cancer by targeting miR-524-5p-METTL3/SOX2 axis Hongya Xie , Jie Yao , Yuxuan Wang & Bin Ni Pages 1257-1271 Received 24 Jan 2024, Accepted 14 Mar 2024, Published online: 25 Apr 2024 Download citation irc fax numberWebFeb 28, 2024 · CCK8 was used in ( a) A549 cells and ( b) H460 cells with different concentrations of cisplatin. ( c) CCK8 was used in A549 and H460 cells transfected with miR-494 or controlled with... order by in athenaWebMay 27, 2024 · Uncovering the role of p62 in the development of cisplatin resistance in ovarian cancer. ... (IHC) and its proliferation and invasion effects via CCK8 and transwell assays. Additionally, expression of cyclin D1, MMP2, p‐ERK, and EMT‐related proteins was measured by western blotting. We found that SIX1 had significantly higher expression in ... irc fb3WebMay 28, 2024 · Non-Small Cell Lung Cancer (NSCLC) patients harboring EGFR activating mutations, such as in-frame deletions in exon 19 (Ex19del) or missense mutation in exon … irc fellowshipWebCisplatin is a platinum-based agent whose nephrotoxicity is thought related to the chloride in the cis position. Cisplatin gains entry into tubular cells via uptake by the OCT-2 … order by importance